How hybridisation and allopolyploidisation affect the diversity in three Brassicaceae tribes (GAČR – Czech Science Foundation; 2019-2021)

The project deals with the role of hybridization and allopolyploidy in generation of plant diversity on the examples from the three Brassicaceae tribes. We will address genomic, phenotypic and ecological consequences of within- and across-ploidy crossing and assess its relevance for plants growing in their native environment. Multiple methodical approaches, namely genomic techniques (RADseq, Hyb-Seq, enabling targeted sequencing, microsatellite markers), genomic in situ hybridization (GISH, FISH and chromosome painting - CCP), DNA content (genome size) measurements, multivariate morphometrics, artificial hybridization, ecological and climatic niche modelling will be used. Important advantage is the detailed knowledge of the plant systems by the team, including their evolutionary history, experience with proposed methods in the studied genera and already available plant material for DNA analyses. Close relationships of the studied taxa with Arabidopsis thaliana makes the use and interpretation of genomic techniques easier and grants the wide publicity of acquired results.

Pathways and consequences of polyploidy and chromosomal variation in plant evolution - three cases from the model family Brassicaceae (GAČR – Czech Science Foundation; 2016-2018)

The project deals with triggers and consequences of polyploidisation (whole genome duplication) on the example of three polyploid complexes of Brassicaceae. Analysis of the influence of cryptic variation of diploid and independently arisen polyploid lineages, together with ecological and morphological consequences of polyploidisation, and influence of selection on genomes of di- and polyploid lineages is carried out. For the Cardamine pratensis complex, evolutionary potential of dysploidy is also estimated. Advanced genomic techniques (RADseq, target enrichment, genome resequencing), comparative chromosome painting (CCP), ecological experiments and bioclimatic modelling are used. Key advantage is the detailed knowledge of all three plant systems by the team, including details on evolutionary history, experience with proposed methods in the studied systems and already available material for DNA analyses.

Arabidopsis arenosa  - photo M. Kolník