Article details
| Title: | Preparation and preservation of nuclei from plant tissues for quantitative DNA analysis by flow cytometry |
|---|---|
| Authors: | Hopping, M. E. |
| Countries: | NZ - New Zealand (Aotearoa) |
| Year: | 1993 |
| Publication: | New Zealand Journal of Botany |
| Pagination: | 31:391-401 |
| Family: | Actinidiaceae, Poaceae, Fabaceae |
| Species: | Actinidia deliciosa var., Hordeum vulgare, Vigna radiata, Zea mays |
| Plant Group: | Angiosperms |
| Objectives: | Genome size |
| Sub-objective: | Preservation methods |
| Type of Standardization: | Internal |
| Type of Standard: | Plants |
| Plant standards: | 1 - Vigna radiata cv. Berken, 2 - Zea mays cv. Va35 |
| Standard 2C values: | 1 - 1.05, 2 - 5.16 |
| Fluorochromes: | Propidium iodide |
| Buffers: | Galbraith |
| Buffer modifications: | nuclei fixed in glycerol |
| Flow Cytometer: | Beckman Coulter - EPICS Profile II |
| Histograms: | Y |
| Herbarium Vouchers: | |
| Plant DNA C-values Database: | N |
| CV range: | 0-3% - 5-10% |
| Date added: | 2008-05-05 |
FLOWer - A Plant DNA Flow Cytometry Database © 2007-2008
code by Oleg Kovářík