Article details

Title: Comparison of plant DNA contents determined by Feulgen microspectrophotometry and laser flow cytometry
Authors: Michaelson, M. J.; Price, H. J.; Ellison, J. R.; Johnston, J. S.
Countries: US - United States
Year: 1991
Publication: American Journal of Botany
Pagination: 78:183-188
Family: Malvaceae, Asteraceae, Solanaceae, Fabaceae, Poaceae
Species: Gossypium hirsutum hybrids, Helianthus annuus, Lactuca sativa, Lycopersicon esculentum Hybrid., Microseris douglasii, Pisum sativum, Sorghum bicolor, Triticum aestivum, Vicia faba, Zea mays
Plant Group: Angiosperms
Objectives: Inter-technique comparison
Sub-objective:
Type of Standardization: Internal
Type of Standard: Plants
Plant standards: 1 - Hordeum vulgare cv. Sultan
Standard 2C values: 1 - 11.12
Fluorochromes: Propidium iodide
Buffers: Galbraith
Buffer modifications:
Flow Cytometer: Ortho - 50-H
Histograms: Y
Herbarium Vouchers:
Plant DNA C-values Database: S - 229
CV range: 0-3% - 3-5%
Date added: 2008-05-05

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