Article details
| Title: | Visual selection and maintenance of the cell lines with high plant regeneration ability and low ploidy level in Dianthus acicularis by monitoring with flow cytometry analysis |
|---|---|
| Authors: | Shiba, T.; Mii, M. |
| Countries: | JP - Japan |
| Year: | 2005 |
| Publication: | Plant Cell Reports |
| Pagination: | 24:572-580 |
| Family: | Caryophyllaceae |
| Species: | Dianthus acicularis |
| Plant Group: | Angiosperms |
| Objectives: | Ploidy level |
| Sub-objective: | In vitro culture |
| Type of Standardization: | No standardization |
| Type of Standard: | No standardization |
| Plant standards: | |
| Standard 2C values: | |
| Fluorochromes: | DAPI |
| Buffers: | TrisMgCl2 |
| Buffer modifications: | Partec --> Two steps kit, Mishiba staining solution composition |
| Flow Cytometer: | Partec - PAS |
| Histograms: | Y |
| Herbarium Vouchers: | |
| Plant DNA C-values Database: | N |
| CV range: | 0-3% |
| Date added: | 2008-05-05 |
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