Article details
| Title: | A simple protocol to purify fresh nuclei from miligram amounts os meristematic pea root tissue for biochemical and flow cytometry applications |
|---|---|
| Authors: | Chiatante, D.; Brusa, P.; Levi, M. |
| Countries: | IT - Italy |
| Year: | 1990 |
| Publication: | Physiologia Plantarum |
| Pagination: | 78:501-506 |
| Family: | Fabaceae |
| Species: | Pisum sativum |
| Plant Group: | Angiosperms |
| Objectives: | Technique |
| Sub-objective: | |
| Type of Standardization: | No standardization |
| Type of Standard: | No standardization |
| Plant standards: | |
| Standard 2C values: | |
| Fluorochromes: | DAPI |
| Buffers: | Chiatante |
| Buffer modifications: | nuclei fixed in glycerol |
| Flow Cytometer: | Leitz - MPV |
| Histograms: | Y |
| Herbarium Vouchers: | |
| Plant DNA C-values Database: | N |
| CV range: | 3-5% - 5-10% |
| Date added: | 2008-05-05 |
FLOWer - A Plant DNA Flow Cytometry Database © 2007-2008
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