Article details

Title: Comparison of four nuclear isolation buffers for plant DNA flow cytometry
Authors: Loureiro, J.; Rodríguez, E.; Dolezel, J.; Santos, C.
Countries: CZ - Czech Republic/PT - Portugal
Year: 2006
Publication: Annals of Botany
Pagination: 98:679-689
Family: Crassulaceae, Fabaceae, Oxalidaceae, Poaceae, Solanaceae, Ulmaceae
Species: Celtis australis, Festuca rothmaleri, Lycopersicon esculentum Hybrid., Oxalis pes-caprae, Pisum sativum, Sedum burrito, Vicia faba
Plant Group: Angiosperms
Objectives: Genome size, Technique
Sub-objective:
Type of Standardization: Internal
Type of Standard: Plants
Plant standards: 1 - Lycopersicon esculentum cv. Stupicke
Standard 2C values: 1 - 1.96
Fluorochromes: Propidium iodide
Buffers: Galbraith, LB01
Buffer modifications: Pfosser
Flow Cytometer: Beckman Coulter - EPICS XL
Histograms: Y
Herbarium Vouchers: N
Plant DNA C-values Database: N
CV range: 0-3% - 5-10%
Date added: 2008-05-05

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