Target enrichment for plant/animal systematics - methodological workshop
7^th - 16^th June 2023

Roswitha Schmickl, Tomáš Fér, Vojta Zeisek, Luciana Salomon

Department of Botany, Charles University, Prague
Contact, registration: roswitha.schmickl@natur.cuni.cz

Course schedule is here

Day 1
General introduction, wet lab (library preparation) - presentation

Day 2
Wet lab - basic protocol, NEBNext Ultra II protocol, video, SPRI beads size selection protocol
Paper discussion - Ufimov et al. 2021
Universal vs. custom probes - participant´s presentation

Day 3
Target enrichment principle - presentation
Participant´s paper presentation

Day 4
Computer work with the data (HybPiper etc.) - presentation
Gene trees vs. species tree - presentation

Day 5
Gene trees vs. species tree - presentation (continue)
Computer work with the data - R code

Day 6
Computer work with the data - HybPhyloMaker - presentation, rapid instruction (PDF or txt)

Day 7
Plastome assembly - presentation, HybPhyloMaker with chloroplast data (instructions), how to run FastPlast on MetaCentrum (txt)
Cytonuclear discordance - paper for discussion
Hyb-Seq vs RADseq vs hyRAD - participant´s presentation

Day 8
Wrap up, varia, hands-on session with own data

Thank you for participating ...